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Oral transplantation of WT germ-free mice with fecal microbiota from Nlrp6 -/- mice attenuated CS exposure-induced lung inflammation To confirm that gut microbiota regulate airway inflammation from CS exposure in an NLRP6-dependent manner, we colonized germ-free (GF) WT mice (WT GF ) with fecal microbiota obtained from WT or Nlrp6 -/- mice bred in the same EOPS mouse facility

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